OSMOSIS IN SIMULATED CELLS
1. Access the “cell homeostasis simulation” at the following website:
2. Press the “START” button and follow the steps of the simulation to set up 5 beakers.
3. When directed to “record in your lab notebook”, write the correct concentrations of sugar on the beakers in Figure 1 to record the solutions in the beakers (before the cells are added).
Figure 1: Five beakers from osmosis simulation.
4. Press the “CONTINUE” button. Complete table 1 and table 2 (in the Results section page) for each of the beaker set-ups as you are building them online.
Table 1: Summary of the osmosis simulation.
Cell (dialysis tubes) Contains:
(use % solute)
(use % solute)
5. Before you continue in the simulation, make a hypothesis and identify the variables in this experiment.
Make a hypothesis about the net movement of water for each beaker. Please note that the sugar in this simulation cannot move across the dialysis tubing. Indicate your hypothesis by adding an arrow to each of the beakers in Figure 1. Indicate the net movement of water by pointing the arrow into the cell, out of the cell, or use a double headed arrow to indicate no net movement of water.
Figure 2: Five beakers and cells from osmosis simulation.
Correctly identify the following variables:
Dependent Variable: _______________________________________
Independent Variable: _______________________________________
3 Controlled Variables: _______________________________________
Confounding Variable: _______________________________________
6. Press the “CONTINUE” button. Record the mass of each dialysis tube as you continue the online simulation. Record these masses at the start (from step 4), and then after “24 hours” in Table 2 (in the Results sections). Calculate the change in weight for each, including a “+” sign for tubes that gained weight, or “-“sign for those that lost weight.
7. OPTIONAL- Press the “CONTINUE” button to answer a few questions.
Fill in the table from procedure # 4 & procedure #6.
Table 2: Weight of cells (dialysis tubes) from before and after simulation.
Weight of Cell (dialysis tubes) at start
Weight of Cell (dialysis tubes) at end
Change in weight
1. Graph the CHANGE IN WEIGHT for each of your simulated cells from Table 2 (in the results section). Make certain that you provide an appropriate Figure description, axis labels, units of measurement, and accurately graphs the data points you obtained.
2. Review the hypothesis you made in procedure # 5, then compare the hypothesis to the results.
For each of the five simulated cell/beaker combinations explain what happened using appropriate terms (concentration gradient, osmosis, hypertonic, isotonic, hypotonic, diffusion, and selectively permeable membrane.)
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